Isolation and identification of a coronatine producing strain

Abstract:

Abstract: [Objective] We screened and isolated coronatine-producing stains from various samples. [Methods] The strains were isolated and selected from samples by the methods of streak plate and serially dilution. The samples were sick leaves/branches and soil in which plants got sick according to the symptoms of leaf blight disease and tuber enlargement. Coronatine production was determined by high performance liquid chromatography (HPLC). The strain was characterized by the physiological and biochemical analysis, the determination of (G+C) mol% contents and 16S rDNA sequencing. The molecule structure of the fermentation product was identified based on the data of ultraviolet spectrum, infrared spectrum and mass spectrum. [Results] Strain BCC933 was gram-negative, polar flagella, short rod and non-spore-forming bacterium and accumulated poly-β- hydroxybutyrate (PHB). It producted catalase, but not arginine dihydrolase nor oxidase, couldn’t grow at temperature 41℃. It hadn’t the abilities to hydrolyze starch and gelatine. No nitrate reduction and denitrification activity was detected. The (G+C) mol% content was 67.2%. We analyzed 16S rDNA nucleotide sequence, and ascertained the phylogenetic position of the strain. [Conclusion] Strain BCC933 with coronatine biosynthesis ability was identified as Burkholderia cepacia, which hasn’t been reported up to date.