Detection of pathogenic Vibrio by Smartongue

Abstract:

Abstract: [Objective] Electronic tongue is a modern analysis instrument and can detect very well the comprehensive information of liquid samples. Based on this, we employed the electronic tongue to detect the bacteria liquid culture, with the purpose of developing a new rapid method for the detection and identification of food-borne pathogens.[Methods] A novel voltammetric electronic tongue, smart tongue, was used to detect and differentiate 11 species of pathogenic Vibrio. Principal component analysis (PCA) was used to extract the relevant information obtained by the smart tongue. From the data evaluation, the score plots of PCA were obtained. [Results] According to the plots, we chose the most feasible working electrodes at the most favorable frequency segments were determined for our purpose. The results showed that the best electrode arrays and frequency segments to differentiate pathogenic Vibrio were titanium electrode in 100 Hz, silver electrode in 100 Hz and tungsten electrode in 1 Hz and 10 Hz, respectively. We differentiated 11 species of pathogenic Vibrio independently in the score plot of tungsten electrode in 1 Hz. We also distinguished all the Vibrio with the combination of two of other three electrodes and frequencies. [Conclusion] Smartongue could differentiate well 11 species of pathogenic Vibrio from the results analysed by PCA. It has a promising future as a novel modern rapid analytical technology for detecting and distinguishing the pathogenic Vibrio. The method and results could be a good stencil-plate to the research of detecting the other food-borne pathogens with smartongue, and good reference information for the further study.