Construction and characterization of recombinant Salmonella enterica serovar choleraesuis vaccine strain expressing heterologous antigen of Pasteurella multocida toxin
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Supported by the Doctor Foundation of Henan University of Science and Techoolgy (No. 090001337) and the National High-tech Research and Development Programs of China (No. 2006AA10A206)

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    Abstract:

    Abstract: [Objective] This study was aimed to construct and characterize Salmonella vaccine strain C500 expressing the recombinant Pasteurella multocida toxin (PMT) antigen by the Asd+ balanced-lethal host-vector system. [Methods and results] The DNA fragment encoding C terminal of PMT was cloned downstream from the beta-lactamase signal sequence in the multicopy Asd+ pYA3493 vector to create pYA-PmtC. Fermentation patterns, serotype, and mean generation time of the vaccine strain C500 harboring pYA-PmtC (named with C501(pYA-PmtC)) were identical to those of the parent strain C500. The recombinant pYA-PmtC plasmid was very stable in C501(pYA-F1P2), which expressed secretorily a large amount of the recombinant PMT antigen (named with rPmtC). The virulence of C501(pYA-PmtC) with LD50 of 8.5×106 CFU was a little lower than C500 with LD50 of 4.4×106 CFU based on the method of Reed and Muench. All piglets inoculated with C501(pYA-PmtC) or C500 survived, and had no signs of disease during the entire experimental period. No significant differences were found between these two groups. [Conclusion] The recombinant vaccine strain C501(pYA-PmtC) had a series of biological characteristics silimar to the parent vaccine strain C500. It is likely that C501(pYA-PmtC) could be adapted to develop multivalent recombinant Salmonella vaccine against both infections with S. enterica serovar Choleraesuis and toxigenic P. multocida.

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Zhanqin Zhao, Chen Wang, Ke Ding, Xiaofeng Li, Bin Wu, Shun Lu, Chunjie Zhang, Xiangchao Cheng. Construction and characterization of recombinant Salmonella enterica serovar choleraesuis vaccine strain expressing heterologous antigen of Pasteurella multocida toxin. [J]. Acta Microbiologica Sinica, 2010, 50(1): 91-97

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  • Received:July 03,2009
  • Revised:September 09,2009
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