Molecular Characterization of the early phase of the Novel influenza A H1N1（2009） Viruses

Abstract:

Abstract: [ Objective ] Pathogens of the first influenza pandemic this century belong to influenza A H1N1 viruses, which are different from human seasonal H1N1 viruses in antigenic and genetic characterization. To better understand the genetic characteristics and evolution, timely detect variant strains with epidemiological importance, we analyzed in detail the molecular characterization of the early influenza A H1N1 (2009) virus. [ Method ] genomic sequences of reference influenza viruses were obtained from Influenza Resource Center of GenBank. Sequences were analyzed using the EditSeq and Megalign program with the Lasergene sequence analysis software package (DNAStar, Madison, WI, USA). A/California/07/2009 (H1N1) was selected as a representative strains of the novel influenza A H1N1 (2009) virus, and its molecular characteristics was determined. [ Results ] A/California/07/2009 do not contained the molecular characteristics of highly pathogenic influenza virus, and its 11 proteins retained most of the molecular characteristics of swine influenza virus, but also had some characteristics of avian and human influenza viruses. With a classical swine H1N1 and human H1N1 dual character, PB1-F2 protein of A/California/07/2009 terminates after 11aa, 57aa and 87aa，which is a unique molecular characteristics of influenza H1N1 (2009) virus. [ Conclusion ] This is the first report for detailed analysis of Molecular characteristics of the novel influenza A H1N1 (2009)virus. As the virus further adapt and persist in human populations, its molecular characteristics will change accordingly. So we should pay special attention to the effect on virus transmission and pathogenesis.