Effect of tillage and fertilization on bacterial community structure in different depth of black soil

Abstract:

Abstract：[Objective] To analyze the impact of tillage and fertilization on bacterial community structure under different depth in black soil, [Methods] We used denaturing gradient gel electrophoresis (DGGE) profile analysis of bacterial community based on the 16S rRNA gene combining soil physico-chemical characteristics. [Results] DGGE profiles showed that tillage and fertilization influenced more on bacterial community structrue in the surface soil than the subsurface and deep soil, and the divergence distance between the tillage soil and the control was about 4%. Subsurface and deep soil affected less compared with the control, and the divergence distance was 2%. The vertical similarity of bacterial community structure between subsurface and the deep soil was higher than that of suface soil. [Conclusion] Tillage and fertilization affected the surface bacterial community (0?30 cm), whereas little on the deeper soil (>100 cm) bacterial community.