Advances in metabolic engineering of yeast for succinic acid production

Abstract:

Succinic acid is an important four-carbon dicarboxylic acid widely used in the food, pharmaceutical, and chemical industries. Compared with petrochemical-based chemical synthesis methods, microbial fermentation offers an economical and environmentally friendly alternative for succinic acid production, presenting significant potential for industrial applications. Due to the robust environmental tolerance, yeast cell factories for succinic acid production have gradually become a research focus. This review centers on succinic acid production in yeast, providing an overview of metabolic engineering and regulatory strategies for constructing yeast cell factories. The research hotspots in this field include the development of succinic acid biosynthetic pathways, optimization of cofactor supply, and modification of transmembrane transport systems. Additionally, recent advances in cost-effective succinic acid biosynthesis and approaches to enhance yeast strain robustness are discussed. Finally, the review provides the prospects of yeast in succinic acid biosynthesis.