Abstract:Streptomycetes are well known for their dramatic production capacity of antibiotics, which are widely used in medicine and pharmacy. It is the largest and most diverse branch of the phylum Actinomycetota. After years of in-depth research, streptomycetes have made great progress in the field of systematics, culture-dependent diversity research, and natural product resource exploration. Based on these three aspects, this paper reviews the most recently advances, opportunities and challenges in the related studies on streptomycetes, and also proposes the future directions.