基于act序列中国西部披碱草属植物所带内生真菌的系统演化

Phylogeny of Neotyphodium endophyte from western Chinese Elymus species based on act sequences

摘要:

摘要:【目的】研究中国西部披碱草属植物(Elymus spp.)所带无性世代内生真菌的系统进化关系。【方法】克隆15条分离于中国西部披碱草属植物无性世代内生真菌肌动蛋白基因(act)序列，并与下载自GenBank中内生真菌的act序列共同构建系统进化树和网络结构拓扑图。【结果】供试无性世代内生真菌act序列均为单拷贝基因。系统进化关系表明，它们与分离于北美洲披碱草属植物的有性世代内生真菌Epichlo elymi存在不同的起源，而与分离于北美洲直芒草(Achnatherum eminens)的无性世代内生真菌Neotyphodium chisosum具有较近的亲缘关系。进一步研究发现，由北美洲直芒草无性世代内生真菌N.chisosum形成的单元型Hap 8与本研究菌株所组成的单元型Hap 3之间可能存在基因流。

Abstract:

Abstract:［Objective］ We analyzed phylogenetic relationships between asexual endophytes isolated from western Chinese Elymus species.［Methods］ Fifteen act sequences were cloned from asexual endophytes of the western Chinese Elymus species．The phylogenetic tree and network topology structure were constructed using act sequences obtained and Genbank.［Results］ The act sequences from asexual endophyte were single-copy gene．The western Chinese asexual endophye (Neotyphodium spp.) has a different origination from the North American sexual endophyte (Epichlo elymi) from Elymus species，but there is a closer relationship between Neotyphodium spp. and Neotyphodium chisosum from North American Achnatherum eminens．Further，endophyte gene-flow exists between sexual Neotyphodium chisosum from North American Achnatherum eminens (Hap 8) and asexual Neotyphodium sp．from western Chinese Elymus species (Hap 3)．