飞龙斩血内生菌种群分布及抑菌活性检测

Population distribution and antimicrobial activities of endophytes in Toddalia asiatica

摘要:

摘要:【目的】研究药用植物飞龙斩血内生菌的种群组成及其抑菌活性，以了解飞龙斩血内生菌的种群分布状态和得到抗菌活性菌株。【方法】采用严格的表面消毒程序、添加抑菌剂的方法分离、培养内生菌株。利用表型和分子技术相结合的方法对内生菌进行分类鉴定。纸片扩散法对分离获得的内生菌进行抑菌活性试验。【结果】从飞龙斩血植株内分离得到了3株内生细菌，1株内生放线菌和82株内生真菌。分类鉴定为14目16科27属，镰孢属(Fusarium)、拟盘多毛孢属(Pestalotiopsis)、曲霉属(Aspergillus)为飞龙斩血内生真菌中的优势种群。通过对30株病原指示菌的抑菌活性检测，18株内生菌对多种指示菌有明显的抑制作用。16株是内生真菌，分属11属。【结论】本试验研究了飞龙斩血内生菌的种群分布，获得了一些具有抗菌活性的内生菌，为飞龙斩血内生菌资源的开发利用提供了基础。

Abstract:

Abstract:［Objective］To study the population composition and antimicrobial activities of endophytes in medicinal plant Toddalia asiatica．［Methods］Endophytes were isolated from T．asiatica by using an exterior sterilization method，in combination with adding antimicrobial agents．Endophytes were classified and identified by morphological and molecular characters．Antimicrobial activities of endophytes were measured by using paper disc diffusion method．［Results］Three strains of endophytic bacteria，one strain of endophytic actinomyces and 82 strains of endophytic fungi were isolated from T．asiatica． Fusarium，Pestalotiopsis，and Aspergillus were the dominant populations in T． asiatica．Antimicrobial activities of these endophytes were measured against 30 pathogenic microbes，and 18 strains possess substantial inhibitory activities，of which 16 strains were endophytic fungi belonging to 11 genera．［Conclusion］ Endophytic strains with antimicrobial activities were obtained to explore the application of endophytic resources from T．asiatica．